| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 3 | 1 | Bronte Lawler | 10 | Ripley | 46.60 |
| 4 | 3 | Henrietta Hems Gillson | 10 | Repton | 46.26 |
| 5 | 2 | Isla Moran | 11 | Swadlincote | 46.34 |
| Heat Number - 2 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 5 | Isla Davies | 10 | Chesterfield | 43.12 |
| 2 | 7 | Lucy Powell | 11 | Ripley | 42.33 |
| 3 | 9 | Ada Moorhouse | 11 | Derby Excel | 39.10 |
| 4 | 11 | Elsie Hatton | 11 | Repton | 35.33 |
| 5 | 10 | Ava Carter | 11 | BelperMarlin | 37.04 |
| 6 | 8 | Claudia Roberts | 11 | Long Eaton | 41.13 |
| 7 | 6 | Daisy Bailey | 11 | BelperMarlin | 42.80 |
| 8 | 4 | Skylar Murray | 11 | Ashbourne | 43.74 |