| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 3 | 1 | Scarlett Sampson | 15 | Eckington | 3:12.25 |
| 4 | 3 | Isabella Margiotta | 15 | Ashbourne | 3:10.52 |
| 5 | 2 | Ellie Randall | 15 | Chesterfield | 3:10.84 |
| Heat Number - 2 | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 4 | Sophia Lichfield | 15 | Chesterfield | 3:07.47 |
| 2 | 6 | Isabelle Kimber | 16 | Ripley | 2:58.36 |
| 3 | 8 | Sophie Martin | 16 | Chesterfield | 2:54.93 |
| 4 | 10 | Mabel Walker | 16 | Repton | 2:39.82 |
| 5 | 9 | Connie Rennard | 15 | Repton | 2:43.00 |
| 6 | 7 | Alice Green | 15 | Swadlincote | 2:57.11 |
| 7 | 5 | Eva Lemmon | 15 | DerbyPhoenix | 3:07.16 |