| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Zara Jefferies | 15 | Matlock | 36.83 |
| 2 | 2 | Ella Bourne | 15 | Repton | 36.58 |
| 3 | 3 | Elodie Heron | 15 | Derby Excel | 35.08 |
| 4 | 4 | Miley Aram | 15 | Long Eaton | 33.42 |
| 5 | 5 | Amelia Denham | 15 | Chesterfield | 33.60 |
| 6 | 6 | Emily Akitt | 15 | Eckington | 35.68 |
| 7 | 7 | Ella Ratliff | 15 | Derby Excel | 36.79 |
| 8 | 8 | Isla Sherborne | 15 | BelperMarlin | 36.96 |