| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Ella Ratliff | 15 | Derby Excel | 41.23 |
| 2 | 2 | Jasmine Vaartjes | 15 | Repton | 39.94 |
| 3 | 3 | Ruby Green | 15 | Chesterfield | 39.29 |
| 4 | 4 | Amelia Denham | 15 | Chesterfield | 34.50 |
| 5 | 5 | Elena Quinn | 15 | Ripley | 37.61 |
| 6 | 6 | Teyona Fung | 15 | Long Eaton | 39.88 |
| 7 | 7 | Evie Hendry | 15 | Bakewell SC | 40.38 |
| 8 | 8 | Emily Akitt | 15 | Eckington | 41.44 |